Journal: Scientific Reports

Article Title: Inhibitory effects of Schisandrin C on collagen behavior in pulmonary fibrosis

doi: 10.1038/s41598-023-40631-6

Figure Lengend Snippet: Sch C inhibited the differentiation and migration of TGF-β 1 -stimulated HFL1 cells. ( A ) Viability of cells cultured with different concentrations of Sch C by MTT assay. ( B ) Levels of α-SMA and FN in HFL1cells were measured using immunofluorescence (×200 magnification). ( C , D ) QPCR and western blot were used to measure mRNA and protein levels of α-SMA and FN. ( E ) Fibroblast migration ability determined by Transwell assay (×200 magnification). The data were normalized to the intensity of β-actin and are expressed relative to the value of the CON group. Data were presented as the means ± SD (n ≥ 3). *P < 0.05, **P < 0.01, ***P < 0.001 vs CON group; # P < 0.05, ## P < 0.01, ### P < 0.001 vs MOD.

Article Snippet: Fibroblast cell lines HFL1 (Human Lung Fibroblast) were purchased from the National Collection of Authenticated Cell Cultures and cultured in Ham’s F-12K medium (Boster Biological Technology Co. Ltd, USA) containing 10% FBS (Sijiqing, Tianhang Biotechnology Co. Ltd, Hangzhou, China), incubated in a 5% CO 2 at 37 °C.

Techniques: Migration, Cell Culture, MTT Assay, Immunofluorescence, Western Blot, Transwell Assay

Journal: Scientific Reports

Article Title: Inhibitory effects of Schisandrin C on collagen behavior in pulmonary fibrosis

doi: 10.1038/s41598-023-40631-6

Figure Lengend Snippet: Sch C inhibited the expression of MMP2, MMP9, TIMP1, Col 1A1 and Col 3A1 in TGF-β 1 -stimulated HFL1. ( A ) Relative expression of Col 1A1 and Col 3A1 mRNAs. ( B ) Relative expression of Col 1A1 and Col 3A1 proteins. ( C ) Relative expression of MMP2, MMP9, and TIMP1 mRNAs. ( D ) Relative expression of MMP2, MMP9, and TIMP1 proteins. The data were normalized to the intensity of β-actin and are expressed relative to the value of the CON group. Data were presented as the means ± SD (n ≥ 3). *P < 0.05, **P < 0.01, ***P < 0.001 vs CON group; # P < 0.05, ## P < 0.01, ### P < 0.001 vs MOD.

Article Snippet: Fibroblast cell lines HFL1 (Human Lung Fibroblast) were purchased from the National Collection of Authenticated Cell Cultures and cultured in Ham’s F-12K medium (Boster Biological Technology Co. Ltd, USA) containing 10% FBS (Sijiqing, Tianhang Biotechnology Co. Ltd, Hangzhou, China), incubated in a 5% CO 2 at 37 °C.

Techniques: Expressing

Journal: Scientific Reports

Article Title: Inhibitory effects of Schisandrin C on collagen behavior in pulmonary fibrosis

doi: 10.1038/s41598-023-40631-6

Figure Lengend Snippet: Sch C suppressed the expression of LOX in TGF-β 1 -stimulated HFL1 cells. ( A ) Levels of LOX in HFL1 cells were measured using immunofluorescence (×200 magnification). (B, C) QPCR and western blot were used to measure mRNA and protein levels of LOX. Data were presented as the means ± SD (n ≥ 3). *P < 0.05, **P < 0.01 vs CON group; # P < 0.05, ## P < 0.01, ### P < 0.001 vs MOD.

Article Snippet: Fibroblast cell lines HFL1 (Human Lung Fibroblast) were purchased from the National Collection of Authenticated Cell Cultures and cultured in Ham’s F-12K medium (Boster Biological Technology Co. Ltd, USA) containing 10% FBS (Sijiqing, Tianhang Biotechnology Co. Ltd, Hangzhou, China), incubated in a 5% CO 2 at 37 °C.

Techniques: Expressing, Immunofluorescence, Western Blot

Journal: Scientific Reports

Article Title: Inhibitory effects of Schisandrin C on collagen behavior in pulmonary fibrosis

doi: 10.1038/s41598-023-40631-6

Figure Lengend Snippet: Sch C modulated TNF-α/JNK signaling pathway in TGF-β1-stimulated HFL1 cells. ( A ) The protein expression of TNF-α, JNK and p-JNK was measured by Western blot. ( B ) The protein expression of LOX was measured by Western blot. The data were normalized to the intensity of β-actin and are expressed relative to the value of the CON group. The data were normalized to the intensity of β-actin. Data were presented as the means ± SD (n ≥ 3). *P < 0.05, **P < 0.01 vs CON group; # P < 0.05, ## P < 0.01, ### P < 0.001 vs MOD.

Article Snippet: Fibroblast cell lines HFL1 (Human Lung Fibroblast) were purchased from the National Collection of Authenticated Cell Cultures and cultured in Ham’s F-12K medium (Boster Biological Technology Co. Ltd, USA) containing 10% FBS (Sijiqing, Tianhang Biotechnology Co. Ltd, Hangzhou, China), incubated in a 5% CO 2 at 37 °C.

Techniques: Expressing, Western Blot